Quantitative Analysis of Placental Mitochondrial DNA in Severe Hypertensive Pregnancies: A Cross-sectional Study

Introduction: To address the causes of maternal mortality and morbidity, reproductive health related disabilities is a motto World Health Organisation (WHO). Gestational hypertension constitutes as one main cause for morbidity mortality. Aetiology gestational hypertension- remains mystery. Mitochondrial dysfunction, particularly in trophoblastic cells, could play an important role causing postulated to be associated with oxidative stress, impaired differentiation invasion tissue. Alterations peripheral blood mitochondrial Deoxyribonucleic Acid (DNA) copy numbers have been thought possible biomarker dysfunction disorders induced by stress. Aim: estimate, evaluate compare between placenta severe hypertensive pregnancies normotensive pregnancies. Materials Methods: A cross-sectional study was conducted at Tirunelveli Medical College Hospital, Tirunelveli, Tamil Nadu, India, 25 clinically proven cases pregnancies, period year (August 2020 July 2021). The were collected from Department Obstetrics, containers within two hours delivery frozen liquid nitrogen. Relative quantitative analysis placental DNA (mtDNA), done calculating number value comparing levels nuclear genes viz., BECN1 NEB ND1 ND6 using real time Polymerase Chain Reaction (PCR). Results: higher patients than total 21 (84%) had 100-110 vs only 13 (52%) 100-105 group. difference them statistically significant p-value 0.03. Conclusion: Present findings suggest alterations level mitochondria women hypertension, which may well contribute pathophysiology. Future research should include direct measure whole body function, assays that elucidate potential modifiers this association.